Sentence examples for by a final step from inspiring English sources

The errors induced by the Chebyshev approximation can be eliminated by a final step of one or two iterations with f itself, using the zeros of fN as initial guesses.

The column was washed with 2.5 CV of the same buffer and the proteins were eluted with 5 CV of a 60 150 mM KPO4 linear gradient, followed by a final step of 350 mM KPO4 buffer (2 CV).

Thermocycling conditions were an initial step at 94°C, 40 cycles at 94°C for 1 min, 59°C for 30 s and 72°C for 1 min, followed by a final step at 72°C for 7 min. PCR product were sequenced using Big Dye terminator mixture (Applied Biosystems).

PCR cycling for CDKN1A and CDKN1B was at 94°C for 5 minutes, 94°C 30 secs, 55°C for 30 secs, 72°C for 30 secs for at least 28 cycles, followed by a final step at 72°C for 5 minutes.

with an initial denaturation step at 93°C for 3 minutes, followed by 35 or 40 cycles of 93°C for 20 seconds, 60°C for 20 seconds, and 68°C for 1 minute, followed by a final step at 68°C for 10 minutes.

The amplification conditions were as follows: 2 min of initial denaturation at 95°C, then 40 cycles at 95°C for 30 s, 50°C for 30 s, and 72°C for 1 min. The amplification was completed by a final step of 5 min at 72°C to allow complete extension of all PCR products.

Classification accuracies were improved in a final step by tuning the rules of pixel classification using the posterior probabilities of class membership obtained from the Gaussian mixture model.

Therefore, docking hit lists are commonly rescored in a final step by more rigorous and time-consuming methods to gain a more accurate final list of ranked compounds.

It was followed by a final extension step at 72°C for 10 min and finally held at 4°C. 2  μL of PCR products along with loading dye were electrophoresed on a 2% agarose gel and stained with ethidium bromide.

The first cycle was preceded by a denaturation step of 10 min at 94°C, and the last extension cycle was followed by a final extension step of 10 min at 72°C.

The PCR was programmed as follows: an initial step of 94°C/5 min, then 30 cycles of 94°C/30 s, 53°C 60°C/30 s, and 72°C/90 s, followed by a final amplification step of 72°C/10 min. All PCR products were sequenced directly.