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Protein concentration in the extracts was determined by a dye binding protein assay (Bio-Rad, NY, USA) with respect to a bovine albumin-globulin standard [27].
Protein concentrations were determined by a dye binding assay (Bio-Rad, Hercules, CA).
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Soluble proteins were estimated by a dye-binding method [40].
The protein concentration was obtained by a dye-binding assay and immunoblot was used to evaluate the binding of the panel of mAbs produced.
Prior to analysis, samples were thawed and dispersed by sonication for 1 min, and the protein concentration determined by a dye-binding method (Bradford, 1976).
Sulfated GAG concentrations were measured as previously described [ 38] by a colorimetric dye binding assay using 1,9-dimethylmethylene blue reagent.
Protein concentration of the collected media was determined by Bradford dye binding techniques (a standard Bio Rad assay) using bovine serum albumin as a standard.
Urine protein was measured by the dye binding method using a complex of pyrogallol red and molybdenum acid (QuanTtest Red Total Protein Assay System, Quantimetrix, Redondo Beach, CA).
The mechanism of controlled conversion from right-handed to left-handed has been assayed by various dye binding studies using CD and fluorescence spectroscopy.
Protein concentrations were determined by the dye binding method of Bradford Bradfordd, 1976 ) using the Bio-Rad Protein Assay Kit (Bio-Rad LaBovinerieserum).. Bovine serum albumin (BSA), in concentrations of 0.1 – 1.0 mg mL-1, was used for the standard calibration curve.
Protein concentration in the supernatants was measured by protein dye binding assay (protein assay; Bio-Rad Laboratories). Bio-Rad Laboratories
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