To test if the membrane precursors generated by starvation is a downstream effect of PI3K activation, we performed the lipidation assay with the 100K membrane and compared its sensitivity to PI3K inhibition as well as PI3P occlusion with the 25K membrane which contains the ER-Golgi intermediate compartment that induces LC3 lipidation in a PI3K-dependent manner (Ge et al., 2013).
Nevertheless, an IL-23-targeted therapy could potentially have a downstream effect by limiting the activation of Th17 cells and decreasing expression of IL-17 family genes.
The alkalisation of the apoplast is a downstream effect of signalling processing by MAPKs.
First, we demonstrate that not only a non-coding RNA, but importantly also a presumed downstream effect, represented by a specific DNAme signature, reproducibly predicts cancer outcome.
A way to do so is by examining the downstream effects of a false negative finding with a manual biopsy (i.e., for lymphoma).
By comparing the response profile of genes that were significantly altered in the hyper, hypo and hypo+ groups, we identified a subset of genes that are most likely to be directly regulated by TH rather than by downstream effects.
Therefore, we verified effectiveness in our model by examining the immediate downstream effect of IL-6 signaling systemically (SAA in liver) and locally (SOCS3 in muscle).
It is, therefore, important to identify other genes that could be regulated by HIPPI and the downstream effect of such regulation in cells.
These data suggest that cholesterol can affect wild-type amastigotes by additional downstream effects.
As T cell activation requires receptor signalling, transcription factor activation, gene transcription, protein synthesis and protein trafficking, systemic abnormalities in these physiological processes in schizophrenia can potentially be traced in this model by using downstream effects of stimulation such as proliferation, cytokine production and gene transcription as readouts of cell function.
