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Reactivation experiments were performed by imaging neurons at 100 Hz and stimulating with a single pulse, followed by a double pulse 1 s later as shown in Figure 1.
INa and ICa were elicited by a double pulse protocol: a rectangular step to −40 mV (100 ms pulse from a holding potential of −80 mV) elicited INa (and inactivate it by the end of the pulse), then a rectangular step to 0 mV (250 ms) elicited ICa Trains of depolarizing pulses were applied at 0.1 Hz.
Although some evidence suggests that apoptosis can be triggered by a single injection of 20E in silkworm fat body [ 52], Sakurai and coworkers [ 64– 67] showed that, in the anterior silk gland, cell death is regulated by a double pulse of this hormone, which peaks twice during larval-pupal transition.
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Two types of thin films were obtained using two deposition mode: the first one called thin Mn-Bi layers by using a single applied potential and the second one called Mn-Bi/Bi bilayers by using a double pulse potential.
By applying a double pulse of BrdU at 6 and 18 hr after fusion, virtually all (90%) successfully reprogrammed B cells identified on the basis of Oct4 re-expression, were shown to have undergone DNA synthesis within a day of fusion with ESCs.
Patients commenced treatment by delivering two consecutive pulses (a double pulse), which they repeated after a minimum interval of 15 minutes on treatment days.
Double pulses also occur in numerous other katydid genera [23], [31] suggesting that pulse pattern is a pliable trait with a propensity to evolve a double pulse structure.
Recovery from inactivation was determined with a double pulse protocol consisting of 500 ms prepulses to −20 mV, followed by variable recovery intervals at −120 mV, and a constant 20 ms test pulse to −20 mV at a pulsing frequency of 0.25 Hz (or 0.15 Hz for R1632H only).
Steady-state inactivation was determined with a double pulse protocol consisting of 500 ms prepulses from the holding potential of −120mV (or −140mV for R1632H only) to voltages between −140 and −20 mV (or −160 and −20mV for R1632H only), followed by a constant test pulse of 20 ms duration to −20 mV at a pulsing frequency of 0.5 Hz.
Two earlier studies addressed the question whether joining these phenomena in a double pulse consisting of a HV pulse and a LV pulse is superior to single pulse strategies for eukaryotic cell transfection.
Thus, calls of both N. maxillosus and N. retusus have a double pulse pattern and differ qualitatively from the single pulse calls of N. robustus.
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