Sentence examples for by a double crossover from inspiring English sources

After linearization at the KpnI/SacI restriction sites, the vector could be integrated into the MSP1 locus of P. berghei by a double crossover event.

Plasmid pLox2 was then introduced into strain V19 and subsequently integrated into the genome of this strain by a double crossover event.

This could be explained either by a double crossover or by us failing to detect the CNV.

Following initial ordering, the genotype matrix for each LG was investigated and data points were eliminated where a single locus was flanked by a double crossover.

It seemed possible that a linear plasmid containing two inverted FRT sites, one at each end, might be "healed" by a double crossover between the plasmid and a linear fragment catalyzed by Flp.

As part of the pre-processing of genotype data prior to map integration, we carried out a masking of genotype scores where single data points were eliminated where a single locus was flanked by a double crossover.

MS allelic changes at the Chr 12 locus (gch1) in our data indicate copy number fluctuation by sister chromatid exchange, a double crossover or gene conversion.

The single-crossover was then inoculated at 30 °C for 12 h on LB plate without antibiotic; Kanamycin-sensitive colonies from a double crossover event were verified by PCR using primers slaC-F1 and slaC-R2 to obtain the slaC mutant strain MG12.

Strains resulting from a double crossover event were selected by PCR.

Then, Kmr, sucrose-resistant and chloramphenicol-sensitive isolates in which the loss of pLAW344 by a double-crossover mutation occurred, were selected.

Homologous recombination by a double-crossover event between S. flavoviridis SB9001 and pBS9078 afforded the mutant strain S. flavoviridis 9031, whose genotype of a zbmA-orf38 deletion from the zbm cluster was confirmed by Southern analysis.