Sentence examples for by a direct sequencing from inspiring English sources
AOX1 SNPs were analysed by a direct sequencing method.
The epidermal growth factor receptor mutation status was determined by a direct sequencing method (exons 18 21 in EGFR).
EGFR mutation status was determined by a direct sequencing method and by Scorpion Amplification Refractory Mutation System (ARMS) technology.
The complete genome sequence was determined by a direct sequencing using the products of RT-PCR, 5'RACE, and 3'RACE as sequencing templates [ 10].
The expected amplification product of 277 bp was used for HBV genotyping by means of a direct sequencing analysis using an automated sequencer (ABI Prism 3100, Applied Biosystems, USA).
Ncl has been shown to activate endogenous Bcl-2 and CD34 gene expression in CD34 positive hematopoietic cells by a direct sequence-specific interaction with the CD34 promoter region and is thus involved in the maintenance of these progenitor/stem cells in hematopoiesis [23].
We also confirmed these results by an independent direct sequencing method.
Screening by SSCP was considered appropriate, since we never detected a mutation by direct sequencing after a negative result on the SSCP in a pilot study.
We also confirm the mutation by a direct DNA sequencing in the four breast cancers (data not shown).
Those 121 variants were subjected to validation by a direct Sanger sequencing, and 67 were confirmed to be present in the tumor, but absent from the normal samples.
