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This was followed by a direct sequence analysis of the amplified products.
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Ncl has been shown to activate endogenous Bcl-2 and CD34 gene expression in CD34 positive hematopoietic cells by a direct sequence-specific interaction with the CD34 promoter region and is thus involved in the maintenance of these progenitor/stem cells in hematopoiesis [23].
AOX1 SNPs were analysed by a direct sequencing method.
EGFR mutation status was determined by a direct sequencing method and by Scorpion Amplification Refractory Mutation System (ARMS) technology.
The epidermal growth factor receptor mutation status was determined by a direct sequencing method (exons 18 21 in EGFR).
The complete genome sequence was determined by a direct sequencing using the products of RT-PCR, 5'RACE, and 3'RACE as sequencing templates [ 10].
The expected amplification product of 277 bp was used for HBV genotyping by means of a direct sequencing analysis using an automated sequencer (ABI Prism 3100, Applied Biosystems, USA).
Forty-five patients with esophageal, gastric or colorectal cancer (Table 1) who had developed severe toxicity following chemotherapy regimens based on 5-FU and had been tested for DPYD exon 14 mutations (including the exon 14 skipping mutation IVS14+1G > A) by direct sequencing analysis at the Portuguese Oncology Institute - Porto, Portugal, from January 1994 through December 2008.
We also confirm the mutation by a direct DNA sequencing in the four breast cancers (data not shown).
Those 121 variants were subjected to validation by a direct Sanger sequencing, and 67 were confirmed to be present in the tumor, but absent from the normal samples.
We also confirmed these results by an independent direct sequencing method.
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