The average molecular weight and molecular weight distribution of P AA-b-NIPAAm-b-AA P AA-b-NIPAAm-b-AA P AA-b-NIPAAm-b-AA P AA-b-NIPAAm-b-AA P AA-b-NIPAAm-b-AAsolviat deliveRAFTystem, two columns (Malvern Viscotek T-columns LT5000, Malvern Instruments, Ltd., Taipolymerizationaintained at 40°C, and a differential refractometer (RI).
Molecular weights were measured by size exclusion chromatography (SEC) [gel permeation chromatography (GPC)] on a set DIONEX, series Ultimate 3000 (degasser, pump, autosampler), 4 Shodex OH-pack 30 cm in series (803HQ, 804HQ, 806HQ, and 807HQ), a differential refractometer OPTILABrEX (Wyatt Tech), and a light-scattering detector DAWN multiangle HELEOSII (Wyatt Tech).
A gel permeation chromatography system made up of an HPLC pump (Waters, model 510, Milford, MA), an autosampler/injector (Waters, model 717), and a differential refractometer (Waters, model 410) with an Ultrahydrogel Linear SEC column (Waters, Part No. WAT011545) was used to determine the molecular weights and distributions of the synthesized copolymers.
The specific refractive index increments (dn/dc) value of the HPAM in 0.5 mol L−1 NaCl solution, which was determined by an OPTILAB T-reX differential refractometer (Wyatt Technology) at 633 nm and 25 °C, was 0.162 mL/g.
Glucose, succinate, glycerol, acetate and ethanol were detected by a Waters 410 Differential Refractometer.
Size exclusion chromatography (SEC) measurements were performed with a Shodex differential refractometer RI 71 in DMF with LiBr (75 °C, column PSS GRAL e4/3/2) or chloroform (30 °C, columns PSS SDV e4/3/2 and SDV e9/5/4) as eluent by injection of 150 μl of the polymer solution.
Peaks were detected using a Waters 410 differential refractometer and a Waters 2487 dual wavelength UV (210 nm) detector.
Peaks were detected using a Waters 410 differential refractometer and a Waters 2487 dual wavelength UV (210 nm) detector.
Peaks were detected using a Waters 410 differential refractometer and a Waters 2,487 dual wavelength UV (210 nm) detector and the results processed using Waters Empower software.
SEC-MALS was performed using an ÄKTA Purifier with Superdex 200 10/300 GL SEC (GE Healthcare), with column output fed into a DAWN HELEOS II MALS detector with laser source at 664 nm and eight fixed angle detectors (Wyatt Technology, Santa Barbara, CA), followed by an Optilab T-rEX differential refractometer using 664 nm LED light source at 25°C (Wyatt Technology).
