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For those eukaryotes lacking Srx, some possibilities for how they differ are that the Prx repair function is performed by a different enzyme, that hyperoxidized Prxs are not rescued, and/or that non-stress-related peroxide signaling is either not as important or not similarly regulated by Prx hyperoxidation.
The siting of m6A at the TSS belongs to a different story and represents installation of m6A on the first transcribed nucleotide (part of the 5′ cap structure) by a different enzyme.
This region was shown to be important for the phosphorylase activity of HprK/P, suggesting that in α-proteobacteria HprK/P might not be able to efficiently dephosphorylate P-Ser-NPr or dephosphorylate it by a different enzyme [30], [42].
In ddRAD, a second digest of gDNA by a different enzyme follows the first.
Black elements indicate transformations for which q is a known substrate or product; dark gray elements indicate either different transformations of enzyme e (members of T e, see Section 2.2), or identical transformations t by a different enzyme (members of E t ).
The structure of SnogD, in complex with nucleotide, supports the mechanism for the formation of the O-glycosidic bond, but it is less clear how SnogD catalyses the synthesis of a C C bond, which may in fact be generated by a different enzyme [ 40].
Similar(53)
There are three varieties, each caused by a defect in a different enzyme involved in the breakdown of mucopolysaccharides, a group of substances important in the structure and maintenance of connective tissues.
MPS III is classified into four types (A,B,C and D), with each type caused by a deficiency of a different enzyme.
Furthermore, the time required for the batch hydrolysis step could be varied by choosing a different enzyme-to-substrate ratio.
Furthermore, the same approach can be applied to other biological systems by replacing chitosan with a different enzyme-cleavable polysaccharide.
We use a systems biological approach to analyze the stress imposed by expressing different enzyme variants from a lineage of soluble P450 monooxygenases, previously evolved for heterologous activity in Saccharomyces cerevisiae.
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