Sentence examples for by a detection objective from inspiring English sources
The fluorescence generated within the specimen is collected by a detection objective (Nikon, CFI Apo LWD 25XW, 1.1 NA, 2 mm WD) whose focal plane is co-incident with the light sheet.
The leftmost column shows the illumination speckle patterns behind scotch tape observed by detection objective 1.
The middle and right columns show the fluorescence signal excited with these speckle patterns captured by the detection objectives 1 and 2 respectively.
The images captured by the detection objectives again become blurry due to out of focus fluorescence and the intensity of single beads depends on their overlap with the illuminating speckles.
Z.1 light sheet fluorescence microscope system was equipped with a Carl Zeiss W Plan-apochromat 20x/1.0 detection objective and a Carl Zeiss LSFM 10x/0.2 illumination objective.
The light sheet had to be created within the working distance of 280 µm of the detection objective and was produced by a dedicated illumination objective lens with a high NA in order to achieve a thin light sheet with an axial extension of only 3 µm full-width-at-half-maximum (FWHM).
Therefore, a stack of ∼20 transmission images was taken by sliding the sample through the detection objective's plane of focus.
Detection objective 1 captures a line shaped image.
Detection objective 2 captures a sharp image with negligible out of focus contribution.
The focal plane of the detection objective was imaged onto an sCMOS camera (Zyla, Andor).
Detection objective 1 in that case faces a projection of the light sheet.
