Recently, a partial knockdown of PfCDPK1 was achieved by introducing a destabilization domain into the protein without an obvious phenotype (15).
Activators (TFA) were built by adding a destabilization domain, a Nuclear Export signal (NES), a VP16 activation domain, and a Nuclear Localization signal (NLS).
Recently a very elegant system has been developed that allows the control of the expression levels of a protein of interest by its fusion with a destabilization domain.
These findings seamlessly join many studies on other K+ channels that show a stabilization of the selectivity filter gate by Rb+ (Demo and Yellen, 1992; Zhou and MacKinnon, 2003; Oliver et al, 2004; Cuello et al, 2010a) and a destabilization by Tl+ (Lu et al, 2001; Zhou and MacKinnon, 2003; Oliver et al, 2004; Piskorowski and Aldrich, 2006; Schroeder and Hansen, 2008).
These aggregates facilitate the release of cytochrome c, either by forming pores in the MOM, or by a direct destabilization of the lipid bilayer.
The absence of functional dystrophin or sarcoglycans in the DAPC is accompanied by a strong destabilization of the complex at the sarcolemma (1).
Others accused the prosecutor of being part of a destabilization plan orchestrated by the United States.
In a sense the movie is trying to solve a problem -- the destabilization of American masculine identity, a destabilization that was signaled by 9/11 and the Middle East mire but is also deeply tied to the rise of the Asian economies.
These results indicate that protein unfolding in electrothermal supercharging is predominantly caused by protein destabilization as a result of droplet heating and increasing concentration of destabilizing anions in the ESI droplet, although other factors almost certainly contribute as well.
The effects of ALS-causing variants on the free energy landscape of SOD1 shown in Figure 7 suggest a potential mechanism through which the ALS variants can oligomerize and exert toxicity by either a significant destabilization of the dimer, e.g., A4V, significant destabilization of the monomer, e.g., G93A, or a combination of both events, e.g., L38V and L106V.
