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As the membrane becomes chelate with Co II), the density increases as measured by a density gradient column.
Afterward, the islets were purified by a density gradient Histopaque-10777, Sigmandand then handpicked under a stereo microscope.
The tumor cell enrichment was done by a density gradient centrifugation with the OncoQuick (Greiner BioOne, Frickenhausen, Germany) system.
Tumour cells were isolated from AF samples from two patients (patients no. 2 and 10) by a density gradient separation method.
A leukocytes fraction depleted from epithelial cells was isolated from EDTA-blood by a density gradient centrifugation protocol, largely according to Brandt and Griwatz [ 15].
The spleen lymphocytes were prepared by a density gradient centrifugation on a Ficoll-Hypaque solution (Sigma-Aldrich Co., St . Louis MO, USA).
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Cells were centrifuged by applying a density gradient (UNISEP®; Novamed, Jerusalem, Israel).
Abbreviations: FISH, Fluorescent in situ hybridization; HPV, Human Papillomavirus; PBMC, Peripheral Blood Mononuclear Cells; SB: In order to investigate the molecular phenotype of HPV bearing cells, we enriched samples in round cells by performing a density gradient centrifugation of whole semen.
B cells from six individual donors were isolated from human buffy coats (National Blood Service, Watford, UK) by centrifugation over a density gradient followed by negative selection using magnetic beads.
The mononuclear cells, isolated by centrifugation on a Ficoll-Hypaque, were then separated by centrifugation on a density gradient (1.068 g/ml; Nycodenz, Nycomed AS Oslo, Norway), as described previously [ 18, 19].
CD4+ T cells (purity over 98%) were isolated from peripheral blood by incubation with Rosette Sep Human CD4+T cells enrichment cocktail (StemCells Technologies, Grenoble, France) followed by centrifugation on a density gradient (Lymphoprep, PAA, Pasching, Austria).
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