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Thus, the NRT2 membrane topology is made of two sets of six transmembrane domains connected by a cytosolic loop (Pao et al. 1998; Forde 2000).
They are structurally unusual in that they contain two homologous hydrophobic domains (I and II) each comprising six TM (transmembrane) regions and a pore connected by a cytosolic loop.
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Here we created an activity sensor by inserting cpGFP into a cytosolic loop of the Arabidopsis ammonium transporter AMT1 3.
Topology predictions of PATs, always place the DHHC-CRD domain in a cytosolic loop between TMDs 2 and 3 defined as in Mitchel et al 2006 [14].
Glycosylation studies were utilized to demonstrate that the topology of Pen-2 comprises lumenal N- and C-termini, two TMDs, and a cytosolic loop region.
One region of this loop is positioned close to a cytosolic loop of the NuoA subunit in the membrane domain, and together they seem to be important in keeping the quinone binding cavity intact.
NCX1 is a member of the cation/Ca exchanger superfamily, which is defined by the presence of highly conserved α-repeat regions within 2 membranous hydrophobic domains, which are separated by a hydrophilic cytosolic loop rich in acidic amino acids.
Finally, the identification of a digested N-terminal ~11 kDa fragment suggests that the membrane associated N-terminus is followed by a sterically hindered cytosolic loop at position ~70 100.
TPCs have a unique structure consisting of cytosolic termini, two hydrophobic domains (I and II) each comprising six transmembrane regions and a pore, and a connecting cytosolic loop; however, little is known concerning how these channels are assembled.
The two clusters of TM helices appear to be linked by a roughly 200 amino acid cytosolic loop.
As before, the 3D map showed that the crystals consisted of two stacked membranes connected by facing cytosolic loops of SecY.
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