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More importantly, the images obtained were a high quality that is comparable to those images captured by a conventional microscope.
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It is worth noting that we miss a small fraction of the C-Laurdan spectrum (between 395 415 nm) by using a conventional microscope equipped with a 405 nm laser as the most bluish laser and a detector with a cut-off at 415 nm.
Current standards for the diagnosis of TB using the non-fluorescent Ziehl-Neelsen stain require the screening of upward 100 fields-of-view of ∼180 µm in diameter [21], cumbersome with our system and similarly tedious by eye on a conventional microscope.
The light that was scattered by individual particles was collected using a conventional microscope and spectrally analyzed by a nitrogen-cooled charge-coupled device array that was coupled to a spectrometer[8].
The slides were scored visually using a conventional microscope and grouped by level of positivity into three groups; negative (≤1% stained cells), moderate (1-10%) and high (>10%) regardless of intensity.
Whereas the resolution of a conventional microscope is determined by the optics of the instrument, the resolution achievable with a fluorescence nanoscope depends on both the optics and the properties of the fluorophore.
Furthermore, the limited space-bandwidth product of objective lenses in a conventional microscope can be significantly surpassed by a lensless microscope.
Higher-powered lenses are available, as is an adapter that allows the device to capture images from a conventional microscope.
Considering the fact that a conventional microscope has only 55 μm depth of focus at this magnification level, the increase enabled by the proposed RBL based microscope is over 25 times.
The system combines a conventional microscope, coherent electronics and a Full Frame CCD camera.
The IN Cell Analyzer is about as different from a conventional microscope as a supercomputer is from a PC.
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