Thereafter, microvascular thrombosis was induced by spreading of 25 μl ferric chloride solution (12.5 mmol/l; Sigma) over the cremaster muscle every minute, resulting in a continuous superfusion of the tissue [ 11, 12].
After the signal had reached a constant steady-state value, the Gln was rapidly removed by continuous superfusion.
Additionally, to investigate the reversibility of red cGES-DE5, we observed the response when the applied SNAP was washed out by continuous superfusion (Figure 2E).
The bundle of fibers was then transferred to an experimental chamber, with continuous superfusion of the same solution as in the dissection chamber.
APs in ESCM were studied during continuous superfusion with a solution containing 140 mM NaCl, 5.4 mM KCl, 5 mM HEPES, 2 mM MgCl2, 1.8 mM CaCl2, 10 mM glucose, (pH adjusted to 7.4 with NaOH).
Continuous superfusion (SIF, 30 μl/min) was provided by a calibrated syringe pump (for microdialysis) linked to the tissue with a band of blotting paper.
We then describe an in vitro technique for the continuous superfusion of enzymatically dispersed pituitary cells; this approach is particularly valuable in studying the dynamics of the TSH responses to the factors known (or suspected) to regulate TSH secretion in vivo.
Spinal cords were carefully dissected out from pups under urethane anesthesia (0.2 ml i.p. of a 10% w/v solution) at room temperature, with continuous superfusion with Kreb's solution containing (in mM) the following: NaCl, 113; KCl, 4.5; MgCl27H2O, 1; CaCl2, 2; NaH2PO4, 1; NaHCO3, 25; glucose, 11; gassed with 95%O2/5%% CO2, pH 7.4 at room temperature (22 °C), as described previously.
Confocal imaging was carried out at 34 °C under continuous superfusion with HBSS (pH 7.4) in a chamber mounted on an SP2 Leica confocal microscope.
In control mice, topical application of ANP (continuous superfusion with 100 nM ANP, 30 min) led to a mild, progressive increase in interstitial fluorescence intensity, indicating significantly increased microvascular permeability to albumin.
