Sentence examples for by a construct containing from inspiring English sources

That in the latter constructs only the gene adjacent to the prometer sequence is expressed, was shown by a construct containing a proinsulin gene followed by that for interferon α-2b.

We found that the linker region contributed slightly to self-association as judged by a construct containing both the DBD and linker region (TRF2DBD+L), but the effect was not specific to DNA (data not shown).

Gene knockout cells were generated by sequentially replacing each copy of the RabX1 or RabX2 genes by homologous recombination using first a construct containing the neomycin resistance gene plus ∼1 kb sequences from the 5' and 3' UTRs of the respective genes, followed by a construct containing the hygromycin-resistance marker with ∼1 kb sequences from the 5' and 3' UTRs of the respective genes.

We generated transgenic mice by microinjecting a construct containing a cDNA encoding the Danish mutant form of human BRI 2 to oocytes of C3HeB/FeJ hybrids.

We confirmed this, as well as the fact that UEV-1 acts cell autonomously, by transformation with a construct containing uev-1 coding sequences under the glr-1 promoter (Figure 2B G).

In this work, ES cells were transfected by electroporation with a construct containing a specific genomic sequence harbouring the required mutation, along with the neomycin phophortransferase positive selection cassette (NEO) for selection of positive recombinants, flanked by two homology sequences ("arms") driving the recombination [ 2, 3].

To assess whether oxidative signaling is involved in regulation of nuclear actin filaments induced by MMS, we generated a construct containing the redox-sensing fluorophore roGFP2 fused with 3×NLS roGFP2-NLSS) (Lohmandand Remington, 2008).

We validated the use of the SLC model for ssDNA of varying lengths by subtracting FECs of a construct containing a 70-nt ssDNA site (red) from those of a construct with a 140-nt poly dT) ssDNA site (orange) at each force.

Transgenic mice can be generated by pronuclear microinjection, in which a construct containing the gene of interest (transgene) is microinjected into the mouse oocyte after fertilisation and randomly integrates into the genome, usually in tandem copies.

(B ) Fluorescent imaging of reducing SDS-PAGE gels monitoring multiple turnover fluorescent Ub ligation by full-length Rsp5 (Rsp5FL), or a construct containing all three WW domains plus the HECT domain (Rsp5WW1-3-HECT), in the presence of wild-type or a P107A PPXY motif mutant version of Sna3C.

The synthesis of cyclic DNA was also achieved; a 100-mer oligonucleotide with 5′-azide and 3′-alkyne functionalities was cyclized by addition of CuI to produce a construct containing a single triazole (linkage B, Figure 1).