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The fluorescence signals were quantified by a comparative cycle threshold method.
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thermal cycler with a comparative cycle threshold (ΔΔCt ).
Data was analyzed using a comparative cycle threshold (ΔCt) method [ 32].
The relative gene expression of targets was detected using a comparative cycle threshold method [ 20].
The qRT-PCR data was analyzed using a comparative cycle threshold (ΔCt) method [ 21].
The qRT-PCR data was analyzed using a comparative cycle threshold (∆Ct) method.
The quantification of target gene expression was based on a comparative cycle threshold (Ct) value.
The relative miRNA expression levels were then calculated by a comparative threshold cycle (Ct) method (2−Δct).
Fold changes in expression of each gene were calculated by a comparative threshold cycle (Ct) method using the formula 2− ΔΔ C t). Anti-con and anti-miR-155 were synthesized by RiboBio.
A standard curve was generated for each primer pair as well as for glyceraldehyde-3-phosphate dehydrogenase (GAPDH; using in this case a predeveloped TaqMan assay), to which gene expression levels were normalized by a comparative threshold cycle method.
The hemizygous or homozygous status of transgenic mice was determined by relative real-time PCR, taking a hemizygous DNA as a reference, by using the comparative cycle threshold method [ 15, 33, 34].
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