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It appears that European branch C5c1 is more differentiated, as far as two of three sequenced Polish mtDNAs formed a separate branch (C5c1a), defined by a coding region mutation at np 7694.
The first encompasses eight mtDNAs, and contains a sub-branch defined by a coding region mutation at np 15579 separating the subjects previously described (Agerolese #19 and Cinisara #20 21) from the five new identical mtDNAs found in the Romagnola breed (#22 26).
We have revised the classification of haplogroup M3 that was previously characterized by a coding region mutation 4580 and two control region substitutions 482 and 16126.
Subhaplogroup M3a defined by a coding region mutation at 4580, M4a defined by two coding (6620 and 7859) and three control region (152, 16145 and 16261) mutations.
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The pEGFP-SHP2 WT and C/S mutant were engineered by inserting a coding region into the SalI and BamHI sites of pEGFP vector (Stratagene).
Here, we should remark that the tests based on P values (IUT and MA) take into account the exon length bias directly by assigning a coding region P value with regard to the length meaning that the coding region distribution is built from scored sequences with the same length as those tested (see Supplementary data, Section 3).
At 10% FDR, signals for β-carotene/ β-cryptoxanthin+zeaxanthin) were detected by crtRB1 InDel4, a coding region indel, and SNP ss196501627, with P-values of 2.23 × 10−7 and 3.51 × 10−7, respectively.
PON1's OP detoxification activity is also influenced by enzyme structure, and a coding region polymorphism, Q192R, determines whether glutamine (Q) or arginine (R) is present near PON1's catalytic center (Harel et al. 2004).
FACS is used to isolate parasites in which GFP fuses in-frame into a coding region followed by screening by fluorescence microscopy for those containing GFP targeted to specific intracellular compartments.
The polyglutamine tract is encoded by a trinucleotide repeat in a coding region of the ataxin 2 gene (ATXN2).
Moreover, in all 23 Group 1 VALs, the coding region is represented by five exons, whereas five members of Group 2 VALs have a coding region represented by more exons (7, 8, 9, 10, and 38 exons).
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