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The bacterial colonies surrounded by a clear zone were scored positive (John et al. 2012).
Antibacterial activity was identified and estimated by a clear zone of inhibition in the indicator lawn around the fiber samples.
Yeast colonies that could assimilate PBSA emulsion or oil by producing lipase or esterase were expected to grow on this medium and to become surrounded by a clear zone if the enzyme degraded the PBSA emulsion (Figure 2A).
Positive clones were identified by a clear zone surrounding xylanase-expressing colonies.
Colonies surrounded by a clear zone with a diameter greater than 2 mm in the layer of the indicator culture were considered positive.
The necrotic center was either built by a clear zone (less sever necrosis) (asterisk in Fig. 2b) or by severely necrotic cells (asterisk in Fig. 2c).
Similar(52)
Xylanase activity was found only in MB4 crude extract as shown by formation of a clear zone in RBB-xylan agar medium (Fig. 4c).
The haemolytic reaction was recorded by observation of a clear zone of hydrolysis around the colonies (β-haemolysis), a partial hydrolysis and greenish zone (α-haemolysis), or no reaction (γ-haemolysis).
The enzyme activity was indicated by the formation of a clear zone around the colonies.
The proteolytic activity was detected by the presence of a clear zone which indicated casein hydrolysis.
Proteolytic activity of isolated Bacillus species was detected by the presence of a clear zone which indicated casein hydrolysis.
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