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The synthesis of this protein by a classical solid-phase protocol has already been reported.
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Cortisol concentrations were determined by a direct solid-phase radioimmunoassay using DPC kits (Diagnostic Products Corporation, Los Angeles, CA, USA).
Cortisol concentrations were determined by a direct solid-phase radioimmunoassay and cholesterol and triglycerides by commercial kits.
The samples were further purified by a second solid-phase extraction using Oasis MCX 96-well plates.
Samples were prepared by a solid-phase extraction procedure and were analyzed by liquid chromatography/tandem mass spectrometry.
Cortisol was determined by a solid-phase RIA (DPC, Los Angeles, CA, USA, presently SMSD).
Total and free testosterone were determined by a solid-phase I radioimmunoassay (Siemens Medical Solutions Diagnostics).
ACC was extracted by a solid-phase extraction procedure using half the extract [ 80].
The concentration of oxLDL was analyzed by a solid-phase two-site ELISA (Mercodia, Uppsala, Sweden).
Serum total T4 concentrations were measured by a solid-phase chemiluminescent competitive assayb.
MMP-3 was assayed by a solid-phase EASIA (Bender-Medsystems, Vienna, Austria).
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