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More importantly, the level of HBcAg-specific IFN-γ production could be increased further significantly by a booster immunization with the (HBcAg+MPLA -loaded nanoparticles.
In this case, an LAIV or a DNA vaccine is used as a priming immunization followed by a booster immunization with inactivated influenza virus vaccine (IIV) or protein vaccine.19,20,21,22,23 Here, using the ferret model, we compare a cHA-based LAIV prime IIV boost immunization regimen with an IIV IIV immunization regimen with and without the addition of the adjuvant AS03.
This is followed by a booster immunization with the Profectus recombinant vesicular stomatitis virus (rVSV -vectored HIV vaccine deliverVSV -vectoredlarly.
The primary immunization was followed by a booster immunization in the tail with 100 μg of mBSA (prepared as on day 1 in IFA, if not indicated otherwise, with or without IFN-α) at day 7.
In aged mice, priming with AdC68NP followed by a booster immunization with A/X31 also markedly enhanced frequencies and numbers of NP-specific CD8+ T cells in blood and lungs, which further increased in lungs following A/PR8 challenge.
CIA was induced in male DBA/1 mice by immunization with 50 µL bovine bovine CII emulsified in 50 µL complete Freund's adjuvant followed by a booster immunization in incomplete adjuvant at 21 days.
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Laser pre-illumination enhanced the production of flu-specific antibody by 400% in primary vaccination (Fig. 6A, p<0.01) and by 900% in a booster immunization compared to vaccine control (Fig. 6B, p<0.001).
When immunized with full length AQP4 protein, mice received a booster immunization with AQP4 protein/CFA (in the absence of additional pertussis toxin) after 4 weeks.
Ferrets that received a booster immunization with the cH8/1 LAIV had detectable HI titers against H8 ranging from 20 to 80 (Fig. 3b).
The vaccine revealed potent immunogenicity and effective blocking activity even after a single immunization which became much stronger (approaching 100% reduction) after a booster immunization.
Some animals in each group received a booster immunization of the same strain given 21 days post-vaccination.
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