Lateral stripe melanophores, including those observed along the horizontal myoseptum, were counted by a blinded observer in live progeny from nf1a+/−; nf1b+/− incrosses at 3 and 6 dpf.
Assessment of ultrastructure was performed by a blinded observer.
Myelin, axon diameter, and axon density were determined by a blinded observer.
Clinical signs and laboratory results were obtained once daily by a blinded observer.
Patients were extubated in the intensive care unit by a blinded observer using predefined extubation criteria.
Measurements and Main Results: Time to extubation and postoperative requirements for morphine, midazolam, nitroglycerin, and sodium nitroprusside were recorded by a blinded observer.
Images were reviewed off-line as multiple slices of the raw data or as volume-rendered images by a blinded observer.
Blotting and analysis was performed by a blinded observer.
All animals were examined daily for local and systemic disease by a blinded observer.
Microscope slides with hematoxylin and eosin-stained biopsy tissue were evaluated by a blinded observer.
To count interstitial cells 15 high power fields (hpf, 400×) were analyzed by a blinded observer.
