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All sets of cephalograms were traced at the same time by a blind operator.
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The threshold level was determined by an operator blind to the experimental group to allow positive elements to be included and to exclude background signal.
LacZ staining was observed through a low magnification microscope and described by an operator blind to the genotypes.
LacZ staining and co-expression with NeuN-immunoreactivity (NeuN-ir) was observed by microscopy and described by an operator blind to the genotypes.
And was corrected by a blind woman.
The testing sessions were analyzed by a trained operator blind to the genotype using Observer software (Noldus Information Technology).
Hemorrhages were counted by an operator blinded to the clinical condition of the mouse, and were defined as a well-circumscribed collection of RBCs (10 to 100s) in the brain parenchyma, not associated with a blood vessel.
All experimental data were assessed by an operator blinded to the treatment condition.
The scan was analysed by an operator blinded to the study groups, on an off-line CT workstation.
Four images per culture condition were captured, and Aβ oligomers were quantified manually in five random fields, by an operator blinded to treatments.
All the tissues were then scanned using an Ariol SL-50 scanner (Applied Imaging); all areas of tumor were scored for PTEN loss by FISH status by an operator blinded to the IHC results.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com