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Data were acquired in random order by a blind observer.
The animals were weighed on a daily basis and inspected for clinical signs of experimental autoimmune encephalomyelitis (EAE) by a blind observer as described previously [54].
Histological characterization by a blind observer demonstrated that the small intestine, kidney, and liver sections showed no evidence of tissue injury or inflammation in both groups.
The puncta were identified based on having a fluorescence intensity that exceeded a threshold set by a blind observer to maximize discrimination of puncta from the background, and a diameter between 0.5 and 5 µm.
Upon completion of the 10 minute testing period, the location of the adult was scored by a blind observer as either the EA arm, ambient air arm, or no choice.
Upon completion of the 10 minute testing period, larval location was scored by a blind observer as either the EA arm, ambient air arm, or no choice (defined as the loading arm or any portion of the central chamber); larvae were then placed in separate rearing boxes based upon their choice of arm, and remained there until pupation.
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(e.g. Fig. 1a) Area measurements and volume reconstructions were performed by an experimentally blind observer using a custom computer program kindly provided by R. Wolf and M. Heisenberg, Univ.
Stained slides were reviewed and graded by two pathologists by following a blind protocol (observers had no information on clinicopathologic data).
Each slide was examined by an observer blinded to the diagnosis and clinicopathologic data and reviewed and confirmed by a second blinded observer.
Participants were assessed once by a blinded expert clinical observer with demonstrated excellent reproducibility.
This was video-recorded and quantified by an observer blind to the experimental group.
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