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All morphometric evaluations were performed by a blind analysis.
The rate of vectorial actin polymerization at focal adhesions was determined by a blind analysis (performed from randomly ordered samples by a different person to the one that carried out the experiments and prepared the kymographs) by ImagePro Plus 6.0 software.
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The slides were scored in a blind analysis by two researchers (D.M. and F.B .. HCT15 cells (CCL-225; American Type Culture Collection) were treated with 50 µM sulindac sulfide (Sigma-Aldrich) for 1-24 h and analysed by western blotting following our previously established protocols (Mladenova et al., 2011, 2013).
These properties have been confirmed by the blind analysis of a childhood leukemia dataset.
The boundaries of the tumor area were determined by a neuropathologist via blind analysis of H&E and DAPI stained slides.
Evaluation of protein expression was performed by blind analysis by two observers and discordant cases were discussed in a double-head microscope in order to determine a final score.
The overall healing rate was assessed in a double blind analysis (by CS and GN) of each tendon.
It will be carried out blind analysis by a tester independent from the research group.
In addition, a blind STRUCTURE analysis conducted by combining flies from all nine paired sympatric sites gave a best fit of K = 2 across the PNW, corresponding to the species R. zephyria and R. pomonella (Table S4; Fig. S1 and S2).
31 Nevertheless, we controlled this parameter by matching the exposed group and the controls on age and we conducted a double-blind analysis by two experienced readers.
After three weeks of culture under standard hESC conditions, plates were examined in a double-blind analysis by three independent hESC biologists for iPSC colony formation.
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