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After staining, slides were inspected by a binocular microscope Eclipse 200 (Nikon).
A minimum of six seedlings per treatment were assessed for their root hair density using the photographs taken by a binocular microscope (Olympus SZ40) with an attached digital camera (Nikon; Cool PIX 995) and the experiment was repeated twice.
After 1 min, the cornea and conjunctiva were examined by a binocular microscope with a slit lamp (Topcon SL1E), and each eye was given a score of 0 9 (Norn 1991).
Briefly, under control by a binocular microscope, the blastomeres were rinsed twice in a drop of PBS supplemented with 0.1% polyvinyl alcohol (Sigma-Aldrich, France), using a mouth-controlled, finely pulled glass pipette before being transferred into a transparent microcentrifuge tube containing 3 µl of lysis buffer [Cui et al., 1989].
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The conodonts are studied under high magnification by using a binocular microscope.
Twelve days later, when microfilariae had become L3, the number of protonymphs was evaluated by observation under a binocular microscope.
The resulting fungal material was further purified by hand under a binocular microscope to remove root fragments.
Examination of the external eye and part of the anterior segment is facilitated by the use of a binocular microscope attached to a slit lamp (a variable source of light that projects the image of a slit onto the eye).
From each paraffin block of representative tumour areas, we stained 10 serial sections of 8 μm thickness with nuclear fast red (Sigma-Aldrich, St Louis, MO, USA) and isolated malignant cells by scrape dissection under a binocular microscope.
For tissue preparation, the preserved insects were softened by soaking in 70% ethanol, and dissected under a binocular microscope by using fine forceps.
The size of the beads was analyzed through digital images captured by a digital camera coupled to a binocular microscope and analyzed by the free license software ImageJ, as described in [19].
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