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Uridine was measured by a binary gradient-elution HPLC method.
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The baseline separation of these amino acid derivatives was obtained on a C8 column with a binary gradient elution using the mobile phase of 20 mmol/L mixed acid buffer solution (pH 6.40) and methanol with 2.5% tetrahydrofuran (THF).
C18-scalar (5 μm, 4.6 × 150 mm) Agilent column was used for separation with a binary gradient elution.
A prepacked C18 reverse-phase column (Waters X-Bridge RP-18, 4.6×100 mm, 5 μm) was used for analytical HPLC with a binary gradient elution (solvent A: H2O; solvent B: MeCN) and a flow rate of 1 mL min−1.
The derivatives were separated on a Teknokroma Mediterranean sea C18 column (4,6 × 150 mm; 3 μm particle size) by binary gradient elution.
Elution was performed onto an analytical column (BEH130 C18, Waters) by a binary gradient of buffer A (0.1% (v/v) acetic acid) and B (100% (v/v) acetonitrile, 0.1% (v/v) acetic acid) over a period of 50 min with a flow rate of 400 nl/min.
We conducted reverse-phase chromatography on an Acquity UPLC system (Waters Corporation, Milford, MA, USA) with a C18 column (Waters) and binary gradient elution (20 100% acetonitrile/water for ~ 25 min).
The mobile phase flow was set to 0.45 mL/min with binary gradient elution, using solvent A (aqueous 5% formic acid solution) and B (95% CH3CN, 5% formic acid).
The mobile phase was 0.01 % formic acid in water (solvent A) and 0.01 % formic acid in isopropanol (solvent B) for binary gradient elution.
The binary gradient elution system consisted of 0.001% phosphoric acid in water (A) and 0.001% phosphoric acid in acetonitrile (B).
Moreover, with binary gradient elution using SDS/DOC cosurfactant solution, s-SWCNTs can be further separated according to chirality.
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