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The MRSA252 genomic region that spans these two genes is nearly identical to DNA present in the genome of Listeria monocytogenes, as revealed by a BLASTn alignment (data not shown).
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That process began by performing a Blastn alignment of all the RepeatModeler de novo sequences against the B. tryoni scaffolds (80% identity, e-value 1e-06).
We looked for viral insertions in the DT40 genome by performing a blastn alignment with each of the 5385 viral reference genomes present in Entrez Genomes against the de novo DT40 genome assembly.
Surprisingly, only 448 contigs are identical between the two assemblies based upon results of a BLASTN alignment [30].
Sequence reads were additionally filtered for exclusion of human sequences by both BLASTn alignment at an e-value cutoff of 10-5 and Bowtie2 high-sensitivity local alignment to the human hg38 reference database.
Sequences were compared by using BLASTn alignment search techniques (http://blast.ncbi.nlm.nih.gov).nih.gov
NGS reads were filtered for exclusion of human sequences using Bowtie2 high-sensitivity local alignment to the human hg38 reference database at default parameters followed by BLASTn alignment to all primate sequences in NCBI nt at an e-value cutoff of 10−8 prior to deposition into the NCBI SRA database.
Atlantic salmon reference genome contigs were assigned to linkage groups by Blastn alignment against mapped RAD contigs.
Chloroplast sequences were identified by BLASTN alignment to the S. uncinata chloroplast genome (GenBank accession AB197035) at high stringency (E value smaller than 10-56).
The specificity of primers was evaluated by BLASTn alignment of the primers against the complete predicted transcriptome and the entire genome assembly.
Alternatively, the remaining reads can be aligned on a laptop using BLASTn to just the viral fraction of the NT database, followed by BLASTn alignment of the viral reads to the NT database to verify that they are correctly identified.
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