Kinetic binding data in all cases are adequately and accurately described by a 1 1 binding model, but given the bivalent nature of the IgG (immobilized) and the trimeric state of GP (analyte), the association stoichiometry is likely to be more complex.
Retroanandamide (2f) and its 10 analogues (1a e, 2a e) were synthesized and evaluated for the cannabinoid receptor activation by a [35S]GTPγS binding assay using rat cerebellar membranes, and Chinese hamster ovary cell membranes expressing human CB2 receptors.
For instance, cameleon constructs have been designed based on two GFP protein variants linked by a Ca2+ binding peptide.
Physical interaction between GM1 and Aβ-(1 40) was revealed by a GM1 binding assay using GM1-coated 96-well plates and FITC-Aβ(1 40).
The MyoD gene is also regulated by a Pax3/7 binding site [ 11], although this regulation has not been explored in an embryonic context.
211c Met-lead 1.59 consists of an ECFP with the last 11 amino acids deleted (ECFPΔC11) and cp173Venus FRET pair connected by a Pb2+ binding domain derived from the bacterial metalloregulatory protein PbrR.
The Nef/HCK interaction is mediated by an SH3 binding domain in Nef 72PQVPLR77 which may be too similar with cellular SH3 interaction.
The production of anti-GYL antibody was monitored by an I125 binding assay using viable GYL tumour cells.
Data were analyzed with the BIAevaluation software (version 3.2RC1) by applying a 1∶1 binding model.
First, high relaxivity value in both T1 and T2 achieved by designing a Gd3+ binding site into a stable scaffold protein [18] allows for increased sensitivity in the detection of disease markers by MRI.
