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The reaction products were analyzed by Western analysis using an anti-WRN antibody.
Knock down of MUC1 protein was evaluated by Western analysis using Anti-MUC1* antibody.
Confirmation of this identification was performed by Western analysis using an anti-CaMKKα antibody (Figure 1B).
Corresponding TXNL6 protein levels were also analyzed by western analysis using a TXNL6-specific antibody.
Immunoisolated proteins were detected by Western analysis using anti-GFP antibodies and quantified using the QuantityOne software (Bio-Rad).
TXNL6 transcripts were monitored by RT-PCR and TXNL6 protein examined by western analysis using a TXNL6-specific antibody.
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Next, we tested whether these cells displayed lower expression of Hsp27 by performing western analysis using anti-Hsp27 antibody.
Samples were separated by SDS PAGE followed by western blot analysis using the appropriated antibodies.
We also probed IP3R isoform-expression levels by qRT-PCR and by western blot analysis using isoform-specific antibodies.
The cellular OsUbc13 was detected by western bolt analysis using the anti-hUbc13 mAb 4E11 (Andersen et al. [2005]).
Production of membrane-displayed somatostatin on yeast plasma membranes was confirmed by western blot analysis using anti-FLAG tag antibody.
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