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All of the 5 novel miRNAs tested were detectable by Northern expression analysis.
Of these, 14 families were verified to be genuine miRNAs by Northern expression analysis.
Of the 10 conserved miRNAs tested, 9 were detectable by Northern expression analysis except miR167.
We identified several conserved miRNAs using this approach and at least one novel miRNA which was validated by Northern expression analysis (miRX001; Figure 4).
As expected, we were able to validate several of the conserved miRNA families by Northern expression analysis (Table 2, see below).
Of the remaining 32 candidates, all of the 5 tested were validated by Northern expression analysis (Table 1, see below) suggesting that most of the novel miRNAs identified in the study could be genuine miRNAs.
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Evaluation of their expression by Northern blot analysis revealed increased expression of 16S rRNA after 1 or 2 hr of exposure to H2O2.
Tissue samples were analyzed for interleukin-6 gene expression by Northern blot analysis and for the presence of interleukin-6 protein by immunocytochemistry.
A selection of different human cancer cell lines was screened by Northern blot analysis for expression of Tensin2 mRNA.
Additionally, the level of Tif2 expression by northern blot analysis in tumor 27-186 appeared to be the same as control spleen (Figure 2c).
Each tumor specimen was assayed for TRKA expression by Northern blot analysis and for MYCN amplification status by both fluorescence in situ hybridization (FISH) and real-time quantitative polymerase chain reaction (PCR).
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