After applying a cut-off p-value of 0.01 for multiplicative errors, 1,600 genes remained with reliable expression values.
It is found to have the worst candidate with reliable expression among a set of suitable endogenous reference genes (ERG) in human post-mortem brain when used for the expression analysis of potential candidate genes associated with AD [ 103].
Although the initial workload to isolate and characterise the parent cell lines is equal to that used in the conventional method, the choice of an appropriate marker gene can speed up the screening process and, once generated, such cell lines can be used to generate multiple complementing cell lines with reliable expression characteristics.
Similar qualitative observations of isotherms have been already made in previous works based on a single-phase model, e.g., [10]; however, the present results are more accurate given that a two-phase method is employed combined with a reliable expression for the thermophoresis force.
The gene expression profiles were filtered to select genes with a reliable expression change by applying the criterion of a two-fold change (in the same direction) in expression level in two or more consecutive time points.
With limited coding potential, reliable expression evidence, and strong secondary structure (Fig. 4) this transcript (e.g., GenBank Accession BG472948) was generated from a chromosomal region that aligns at the nucleotide level in several mammals, from human to opossum, suggesting an evolutionary history of at least 170 million years [ 13].
The results show that the platform produces reliable expression measurements with unparalleled sensitivity and precision.
There is thus a need for a Tag-seq method that produces reliable expression data with no or minimal biases and is amenable for analysing hundreds of samples.
Even with reliable gene expression data, statistical analysis of microarray experiments remains challenging to some degree.
A meta-analysis of 11 out 22 functional genomic studies conducted between 2004 and 2009 [ 10] failed to show a gene with reliable differential expression [ 9].
