Human fibroblast 2fTGH cells were stably transfected with these constructs, and 2 cell clones expressing TAP-tagged HuISG15 or TAP-tagged AgmISG15 with comparable expression levels were selected (see Figure S1).
Transduction of these cells with Retro-EF1α-EGFP resulted in EGFP expressing neurospheres that continued to express EGFP for 10 passages (Figure 5, green line) with comparable expression to primary spheres infected at the 10th passage (39.4% and 34.8% respectively, Figure 5, upper right).
The lack of orthology between Drosophila and mammalian lamin genes means that while some important functions are almost certainly performed by lamin genes with comparable expression patterns in different species, this does not necessarily extend to all lamin gene functions.
While the effects of barnase alone on SKOV-3 and BT-474 cells differed 25 times, scFv 4D5-dibarnase demonstrated similar specific cytotoxicity to both HER2-overexpressing cell lines, what agrees with comparable expression levels of HER2 receptor demonstrated immunohistochemically for these cell lines [52].
Those with comparable expression level were selected for this study.
DOSPER, which is another lipophilic medium, has proven to work in serum environments with comparable expression as LipoFECTAMINE in the transfection of cell lines [ 3].
However, if genes with comparable expression levels share the same composition as the intergenic sequences, this would also generate variation in their ICCS RSCU values.
Decreased expression of OPG in SF-derived osteoblasts from patients with pJIA, together with comparable expression of RANKL in both patient groups, resulted in the lower OPG/RANKL ratio in children with pJIA, which might contribute to the increase in osteoclastic bone resorption.
Importantly, similar frequency of monocytes (Fig. S1) with comparable expression of TLR3 (mainly recognize naked poly I C and to some extent poly I C complexed with Lyovec), TLR4 (LPS), and TLR7 (CLO97) (Fig. S2) was observed between age groups; thus, the delayed monocyte response in old individuals was not due to lower TLR expression.
To identify whether groups of miRNAs with comparable expression profiles may be involved in the regulation of specific biological functions, we performed an in silico functional annotation analysis of their predicted target genes using the 25 above- described co-expression sub-clusters (Blue lines in Figure 1A-E and Additional file 1).
