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The model is based on five parameters which are directly measurable but were obtained from literature for the purpose of this study.
These could not be obtained spontaneously in a wild-type background but were obtained from a strain with elevated transcription of the rut operon [ntrB(Con), also called glnL(Con)].
Sequence ages were rounded to the nearest 500 yr BP as most samples were not directly dated but were obtained from well dated stratigraphic layers with a certain age range (Additional file 1: Table S3).
Prices were not collected in the study, but were obtained from nationally representative surveys and data sources, including the Medical Expenditure Panel Survey (MEPS) and the Pharmaceutical Red Book, detailed below.
These cases were not related to the cases presented in Figure 1, but were obtained from an independent source (US Biomax, Rockvile, MD) in the form of tissue arrays, because no tissue slides were available for the same cases as reported in Figure 1.
First, life expectancy estimates on which this analysis is based were not derived from a randomized clinical trial but were obtained from a published analysis that extrapolates annual survival data from 20 observational studies and cohort analyses using Markov modeling and reports life expectancies for three baseline CD4 count categories [ 19].
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Educational information is not part of the UI wage records, but is obtained from a link to the long form of the 2000 Decennial Census.
Simultaneous transmission and emission is not useful for current PET-CT scanners, as the transmission data are not measured with 511 keV but are obtained from a scaling of the CT image.
Since our hospital is a referral centre and many patients return to their private ophthalmologists after surgical treatment, data has not only been collected at our hospital but was obtained from several referring ophthalmologists.
Substate-level data on opioid use is not published, but was obtained from SAMHSA for New York City (NSDUH region A) by the NYC DOHMH (Bureau of Alcohol and Drug Use Prevention, Care and Treatment), which provided the data for this analysis.
Tissues from Peru were not available, but sequences were obtained from GenBank accessions (Table 4).
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