RAR-α, RAR-γ and RXR-α were expressed in all cells but weak expression of RAR-β could be detected in T3M-4 cells only.
cyp19b1 was expressed weakly as early as D21, but weak expression of cyp19a was not detected until at least D47.
SUN719 showed only trace expression of BZLF1, BRLF1 and lytic BHRF1 but weak expression of BLLF1.
In conclusion, we found a widespread but weak expression of Setd8 in SGs, IFE and the HF that correlated well with the occurrence of H4K20me1-positive nuclei, and increased with proliferative phases of the skin.
Correspondingly, frozen RCC tissue samples showed strong expression of GalNAc-T3 but weak expression of GalNAc-T6 (Supplementary Figure 1), whereas other GalNAc-Ts (-T1, -T2, and -T4) were expressed neither in RCC nor in adjacent normal tubular epithelium on frozen samples (data not shown).
The stable line has a more continuous but weaker expression, allowing an equilibrium of γ-complex incorporation and cell localization to be reached, while transient transfection by electroporation leads to an immediate high expression that may not be fully processed by cell machinery before the point of cell lysis.
In healthy liver, IHC confirmed ubiquitous strong expression of D1 protein but a much weaker expression of D3 protein that was mainly localized in stromal cells [ Figure 1C (×10 and ×40) and Figure 1D (×10 and ×40)].
In contrast, three had clearly weaker expression of less than 6%.
In contrast, patient-matched normal epithelial tissue demonstrated predominantly weaker expression of these markers.
The remaining cell lines exhibited a weaker expression of MUC1, which differed significantly from the MCF7 cell line.
