And the study on the wetting behavior of Zn3(phase(H2O)4 phasuggestedthatthet the water wetting property of coating was a key factor for suppressing wax deposition and the weak affinity between coating and wax also played an important role.
However, the assembly could not disassembled completely, which might result from a remnant weak affinity between SsrA and SspB in the dark.
The affinity of 13 kBT corresponds to a mean dissociation constant larger than 1 µM, which is a very weak affinity between proteins and DNA.
As Ernst, et al. suggested, a weak affinity between apo-Fur and the sodB promoter makes physiological sense, as SodB is the only defense H. pylori has against superoxide radical damage [1], [10].
Another study ranked tags in terms of increased expression and yield after purification as GST ~ MBP > CBP > His6 when comparing the expression of 32 human proteins where the molecular weight varied from 17 – 110 kDa.[ 30] Here GST was preferred because of the weak affinity between MBP and amylose resin.
However, they show by EMSA that their Arnt-Max homodimer binds to the E-box with comparable, but weaker, affinity than does the ArntbHLH/PAS domain [21].
The discrepancy may result from weaker affinity between K-loop and L7Ae in the cellular environment compared to in vitro; our system may prove to be an effective way to measure RNP affinity within cells.
The CBM2b-1was shown to bind specifically on xylan and the CBM2b-2 on both insoluble and soluble oat spelt xylan, but exhibited also weak affinity to insoluble cellulose [ 25].
The ER-like binding site has highest affinity for E2 (IC50, 753 nM) but only very weak affinity for T. In addition, BPA exhibits a clear binding (IC50, 24.8 μM).
Fragments are typically <250 Da compounds that bind to proteins with weak affinity but high ligand efficiency.
