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But, these methods require to handle the operations on several coarser levels where the communication costs are higher than the computation costs.
Conventional rule-based algorithms were developed to extract information of SINs, but these methods require engineering knowledge and tedious work for parameter tuning.
Software applying machine learning has become an essential tool to automate analysis, but these methods require annotated examples to learn from.
Modulating the microwave frequency or performing 1H-13C cross-polarization could also improve the polarization level and reduce the build-up time31,32, but these methods require additional hardware.
An optimal policy can be found using existing methods, but these methods require time at best polynomial in the number of states in the domain, where the number of states is exponential in the number of propositions (or state variables).
Different methods such as, isotopic labeling, mass spectrocopy and Nuclear Magnetic Resonance spectroscopy have been used for measuring the level of cellular metabolites or signaling molecules, but these methods require the disruption and fractionation of tissues which suffer from contamination.
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A range of methods using chromatographic separation have been developed in the past [ 8– 11], but usually, these methods require long run times (>50 min) and are not suitable for high-throughput screening and analysis of a large number of plant samples.
Some experts advise killing borers biologically (with the microscopic worms called beneficial nematodes) or chemically (with strong systemic insecticides), but both of these methods require near-perfect timing and ideal conditions.
Recently, several methods have been proposed to overcome this limit but most of these methods require extensive optimization to tailor the protocol to the specific antibody used or number of cells being profiled.
Human cell-free circulating DNA (cf-DNA) derived mainly from cell apoptosis and necrosis can be measured by a variety of laboratory techniques, but almost all of these methods require sample preparation.
During the civil war, the self-appointed witchfinder general Matthew Hopkins tortured suspects by sleep deprivation and other techniques designed to bring about mental collapse; but these methods required teamwork, and as a consequence were never secret.
More suggestions(15)
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