Of course there are more advanced methods to analyze textures, like Laws filter [26], or Tamura measures [27], but these methods need more computational time.
To overcome this issue, numerical methods for NLCS are utilized in [10 14] to generate the perturbation function, but these methods need a large amount of computation, and also, their applicability is limited.
But these methods need sometimes interaction with the user.
Other assays are available, such as free testosterone or bioavailable testosterone measurements, but these methods need further attention to improve their repeatability and availability.
Ultracentrifugation followed by RT-PCR amplification has often been used to obtain these sequences, but these methods need large volumes of plasma, and the extended process results in deterioration of the viral material.
There are many synthetic methods to prepare GQDs [24, 25, 26], but most of these methods need several steps and post-treatment with surface passivating agents to improve their water solubility and luminescence property.
But to be effective, these methods need to be expanded nationwide, Rothstein said.
These methods need special instrument and depend on the experience of the pathologists.
These methods need some parameters such as scanning velocity, soil dielectric, soil conductivity, etc.
These methods need specific selection of appropriate synthesis conditions and suitable surfactants.
