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The staining intensity at these locations varied, but the staining pattern was reproducible.

The localization of tACE3 was examined in Izumo1−/− sperm, but the staining pattern remained normal, resembling that of wild-type sperm (Figure 2).

DiOC also stains mitochondria but the staining pattern of fiber initials indicates most of the staining was ER.

DiOC may also stain mitochondria but the staining pattern was consistent with ER staining, though an increase in mitochondria in these metabolically active cells is also likely.

For Ng, its localization in the nucleus, cytoplasm, and apical dendrite was evident but the staining pattern in basal dendrite within So was patchy.

Except for the smallest W, the W chromosomes were highlighted with the female gDNA probe (Fig.  5k), but the staining pattern of DAPI was indistinctive with only few small heterochromatin blocks of higher intensity (Fig.  5j, arrows).

Similar to our previous reports (Chiu et al, 2001; Chiu and Oleinick, 2001; Lam et al, 2001; Xue et al, 2003b), before PDT cytochrome c was confined to mitochondria, as shown by the perinuclear, punctate staining pattern, but 5 h after PDT, the staining pattern was diffuse throughout the cell, as a result of the release of cytochrome c from the mitochondria into the cytosol.

Other tumor types were more variable in staining pattern, although the staining pattern reliably predicted 99mTc-sestamibi uptake in almost all tumors except chromophobe renal cell carcinoma.

Open image in new window Fig. 3 Visualizing the staining pattern in 3D by FIB-SEM.

The HP1β staining pattern was almost identical to the staining pattern of H3K9Me3.

The staining pattern was different in liver tumours.