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Long period isolation doesn't make the scorpion Lychas mucronatus change their toxin types much in venom, but the primary structure and abundance of these toxin types change a lot which probably related to the adaption of Lychas mucronatus in different environment.
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The spinning disc confocal microscopy data confirmed that fibers were present, but not the primary structure at this time.
We further investigated whether Rab8A Ser phosphorylation could affect GTP hydrolysis since Ser lies close to the functionally important switch II region in the tertiary structure (but not the primary structure; Fig EV3A).
Together with previous structural studies, our results suggest that autoprocessing is dependent on not only the primary structure, but also the peptide flexibility around the processing site.
The three residues are separate in the primary structure but their common participation in binding the small Zn II) ion define their spatial proximity in the tertiary structure of the transporter.
Cliques that consist of three or more residues that are far apart along the primary structure but are in contact in the folded structure are shown to be important determinants of the binding problem.
LMW-GS are distinguished into typical types, namely those proteins having a particular primary structure, and gliadin-like types, namely those LMW-GS that are gliadins according to the primary structure, but functionally act like glutenin subunits, due to the presence of an odd number of cysteine residues.
Linear epitopes consist of amino acid residues that are adjacent to each other in the primary sequence while nonlinear epitopes consist of amino acid residues that are separated in the primary structure but are brought into proximity when the protein is in its native form.
Under this scenario the overall impact on individual fitness of deleterious mutations would be minimized but the shaping by NBDM of the primary structure of many genes would be epiphenomenal and these genes' deleterious mutability under NBDM could appear much less optimized, if at all, were it to be assessed in isolation (see below).
This alignment shows not only the primary structure (sequence), but also the predicted secondary structure elements.
The identification of IUP regions from the primary structure of a protein is thus an important but difficult problem [ 1- 5].
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