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But the expression rate was not quantitatively compared to normal canine mammary epithelial cells.
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The expression rate is low under monolayer conditions, but increased when the cells are under polarising, that is, differentiating conditions.
We therefore hypothesized that the loss of H3mm7 caused the expression rate change of differentiation-related genes and thus the proportion of successfully differentiated cells decreased despite the isogenicity of the H3mm7−/− C2C12 cells.
FACS results showed that the expression rate of CD29 and CD90 in passage 4 MSCs were respectively 71.38% (Figure 1d) and 89.48% (Figure 1e), and the expression rate of CD45 was 0.35% (Figure 1f).
However, in the verification test, the expression rate of YER034W showed the lowest correlation among the three genes.
In addition, the expression rate of PRM5 was significantly increased by heat treatment only in the NFRI3236 strain.
It is a rough first approximation of the expression rate.
The differentiating factor between the AI and AD models described here was the expression rate of PAcP conformers.
Induction timing is very homogeneous across the population, although the expression rate varies somewhat from cell to cell.
The expression rate of mutants L446A, N447A and F451A was less than 60% of the wild type.
However, the expression rate varied between the different transformants.
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