When the machinery required for inducible expression (the T7 RNA polymerase and tetR genes) was placed under the control of an rRNA promoter, the induction profile did not become more homogeneous, but the background expression increased significantly.
MS4A8A was thereby not expressed in the stem cell niche of the crypts, but its expression increased along the crypt luminal axis.
WOX4 was expressed at a relatively consistent level along the length of the inflorescence stem (Fig. 4A); however, WOX14 expression was low at the top but expression increased along the apical-basal axis such that expression was 20-fold higher at the base than at the top (Fig. 4B).
Interestingly, basal expression levels of HRK1 were also very low in the ssk1Δ mutant, but its expression increased at the later time point (60 min) during NaCl treatment (Fig. 2A), indicating that HRK1 expression needs to be induced in response to osmotic shock in the absence of the Ssk1 response regulator, but requires the Hog1 MAPK.
As expected, the Paradoxical group showed no significant induction by TNF in the presence of Dox, but their expression increased 2-fold in the cells stimulated in its absence.
Consequently, mTOR signaling activities were reported to be reduced in FGR placentas [ 81], but the mTOR protein expression increased, suggesting occurrence of adaptive response.
Brachyury gene expression was significantly higher in the NC and NPC + NC groups at day 1 compared with the other groups, but expression increased significantly more in NCs alone than in coculture with NPCs.
The microenvironment of the duct seems to promote glandular differentiation: the transformed cells were predominantly keratin 18 positive/keratin 14-negative while confined to the ducts, but keratin 14 expression increased when these cells migrated into the stroma.
