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But the dose response curves and calculated IC50 values also indicate a general cytotoxic activity.
During isoproterenol stimulation, the force amplitude was comparable at pH levels but the dose response curve was shifted to the right.
The same maximal inhibition of cAMP accumulation was observed, but the dose response curve was shifted approximately 10-fold, with the EC50 for Rog shifting from 0.41 nM to 4.32 nM spiradoline after spiradoline pretreatment.
Both populations responded to 60 minutes of prolactin treatment with the increase in HSP90A mRNA, but the dose response of HSP90A induction in the two-cell population was distinguishable.
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Endothelium-independent vasorelaxation by a nitric oxide (NO) donor, SNP, was attenuated by aging in both WT and GLO1 Tg rats only at the SNP concentration of 10−7 M, but the dose responses were not significantly different between age-matched WT and GLO1 Tg rats (Fig. 3B).
In summary, this study confirms that clodronate is a safe and effective treatment for TIH but failed to confirm the dose response relationship seen in the study of Thiebaud et al (1988).
The other two compounds, NSC359452 and NSC740487, correlated with high Mre11 and Rad50, but further examination of the dose response curves revealed the compounds were only marginally active in HCC2998 and HT29 cells and not in the other cell lines even at the highest concentration tested (10-4 M).
For the basic dose response model, the ERR was assumed to be linear in dose but we also considered models where the dose response was taken as a linear-quadratic, a pure quadratic function of dose, or threshold models in which the ERR was assumed to be 0 up to some threshold dose and taken as linear for higher doses.
Another thing: pharmaceutical researchers are often looking at something they call "the dose response".
The dose response curve is linear.
Several recent assessments by regulatory agencies have used the same study and endpoint, but the dose-response modeling conducted here was more appropriate for the study design.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com