TtMCO has only 39% identity to its closest characterized homologue, CotA from Bacillus subtilis, but sequence and spectrophotometry confirmed copper coordination similar to that of LMCOs.
For the 5-nt-long, 90°-kink loop, the sequence is strictly taken from the HCV IRES domain II.27 For KLs and pRNA loops, the exact sequence is not important, but sequence complementarities are ensured for the interacting pairs.
We have found high variable numbers of repeats in Indian WSSV isolates, but sequence variation within the repeat region was confined to either T or G at position 48.
At the same time, winning three games in a row on command remains the daunting statistical task it's always been – and that gives us our real conflict, because the Cubs' opponent in the second story isn't the Indians or the Dodgers or the Giants, but sequence and consequence, force and pressure: can they do something they've done all year long right now, on demand, in order to save their season?
For enzyme activity classes, many targets were pharmacologically similar, with the higher ligands chemical similarity, but sequence dissimilar [5].
Comparison of primary sequence for the α1- and α2-repeat regions from two CAX proteins and two NCX/MHX proteins demonstrates the sequence similarity within CaCA classes but sequence distinction between them (Fig. 3b).
pil gene synteny is shared between M. xanthus, strain 2CP-C, and G. sulfurreducens but sequence is not as well conserved in G. sulfurreducens (Figure S1A).
But sequence conservation is much higher between the paralogous palindromic arms within a species than between the orthologous sequences across species.
The fluorogenic peptide SNAPtide (FITC/DABCYL, product #521) and the unquenched calibration peptide, containing an N-terminally FITC-labeled fragment of SNAPtide (product #528, synthetic, but sequence identical to the BoNT/A cleaved product), were from List Biological Laboratories.
A few strains were established from a single small colony (consisting of two to five densely aggregated cells enveloped in gelatinous material), but sequence analysis confirmed that these are represented by a single genotype (i.e., clonal).
Genotypic assays are much faster and less expensive than the phenotypic ones, but sequence data provide only indirect evidence of resistance and interpretation is difficult for complex mutational combinations.
