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Alternatives include peptide-based transfection reagents that are highly efficient, but require sonication of the peptide prior to transfection and have not been demonstrated in fully differentiated adipocytes [5].
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The dispersion method does not require sonication, which shortens the SWCNTs and can generate surface defects.
The modified protocol involves the use of a 1 1 mixture of 1% SDS and 8 M urea as protein solubilization solution, and requires sonication.
Sonication is a faster technique, but requires constant operator attention.
This core size decrease (from few micrometers to a hundred of nanometers) for LbL encapsulation required development of sonication assistant non-washing technique and shell PEGylation to reach high colloidal stability of drug nanocarriers at 2 3 mg/mL concentration in isotonic buffers and serum.
In addition, sonication does not require the addition of beads or chemicals, thus decreasing processing cost.
The time required for sonication depends on the fragment size desired as well as on the sonication equipment.
SUVs were prepared by sonication of the required amount of PE PG (7 3, w/w) or PC CH (10 1, w/w).
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