Mr Correa had failed to appreciate the scope of the initiative, which was "not an isolated, one-off technical proposal, but rather a pathway, a transition.
"We want to make sure this isn't simply a rabbit hole, but rather a pathway to achieving our objective," said a senior administration official, who like others requested anonymity to describe confidential deliberations.
It is very important to emphasize that SLEPR is neither a simple extension of GSEA or other group test methods with similar analysis goals nor COPA methods specifically looking for outlier genes, but rather a novel pathway analysis method in terms of its unique concepts and methodology.
The results shown here, together with those published previously [ 24] suggest that early leaf death in old3-1 seedoesgs does not result from activation of a developmental senescence program, but rather a cell death pathway that is related to the plant immune system and stress responses.
Microarray-based gene expression studies have used and abused pathway analysis often calling a pathway what is not a pathway, but rather a gene set and determined a functional enrichment statistics or score based on a pool of genes in the sample (e.g. Fisher exact test, gene set enrichment analysis [ 49]).
It was not viewed as an avenue to health, but rather as a pathway to laziness.
Moreover, by carefully analyzing the time frame required for FGF/SHH effect on hairy2 expression, we conclude that it does not involve a relay mechanism (supplementary material Fig. S4), but rather a convergence of signaling pathways in both time and space.
This, we ascribe to the idea that a defective G1 checkpoint is not necessarily caused by p53 mutations alone but rather a disruption in the p53 pathway, which can be caused by other aberrations within this pathway.
Taken together, the results suggest that Ser phosphorylation is not an on/off trigger mechanism for switching metabolic pathways, but rather a fine-tuned mechanism for carbon allocation within different metabolic pathways.
Surprisingly, we did not find any evidence for a neuroendocrine phenotype (Supplementary Fig S5) but rather a predominance for cell cycle pathways.
The process of aging is probably not induced by the dysregulation of a small number of cellular pathways but rather a complex interplay between subtle changes in a variety of different cellular functions leading to senescence and cellular malfunction with age [ 32].
