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To test potential IFNγ regulation of PD-L expression on DCs during BCG infection, we infected IFNγ KO mice, but observed no differences in PD-L expression compared to wild-type mice six weeks after infection (data not shown).
We performed an exhaustive assessment of Kv11.1 channel kinetics but observed no differences in the rates of activation, deactivation, recovery, fast inactivation and steady-state inactivation parameters following coexpression of Kv11.1-wt and Kv11.1-mut channel constructs (Figure 5 and Table 1).
We also analyzed LC3-II protein level in protein extracts at 4 days post-transduction, but observed no differences between controls and shSmn cells (data not shown).
We assessed the expression levels of the Cu transporters, CTR1, ATP7A, ATP7B, but observed no differences in expression of these proteins between control and CLN6 Merino sheep (unpublished data).
Under these experimental conditions, we observed significant increases in the growth rate and maximum cell growth for the cyto strain (JMY2416) for all carbon sources tested, but observed no differences in the lag phase.
However, as a check we compared the ORs for the exposure variables when both participants were classified first as "white" and then as "other race" but observed no differences.
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We therefore measured the FoxO1 transcript levels in GFP+ crypt cells but observed no difference between DTG and control cells.
We first assessed melanoma cell migration in a wound-healing assay (Rodriguez et al, 2005) but observed no difference in the migration of A375 cells transduced with either control or WLS shRNAs on type I collagen (Supporting Information Fig S5A).
Using N2 worms, we tested whether GE affected transcription of daf-16 and hsf-1, plus a number of daf-16 and hsf-1 transcriptional targets, but observed no difference between GE and non-treated controls (Fig. S9C E).
We tagged these transcripts with EGFP at their N-termini and expressed them in M2 cells with the same transfection efficiency as wild-type FLNa, but observed no obvious differences in subcellular distribution.
We compared rates of participants lost to follow-up in each cohort but observed no significant differences.
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