First we examined localization of Cdc5 in cells treated with cycloheximide, a potent inhibitor of translation but observed no change in the localization of Cdc5 (Figure S4B).
To further test whether epoxomicin alone has an impact on the protein levels of Mfn2, we treated control cells with 10 µM epoxomicin but observed no change in Mfn2 levels during 9 h of incubation (Figure S8).
However, Schodel et al. found a decrease in circulating plasmocytoid DCs numbers but observed no change in the number of myloid DCs [ 8].
We examined the expression of dicer, drosha, ago1, ago2, and trpb2 mRNAs between the β4 and mock transfectants using Affymetrix GeneChip data but observed no change that could account for the downregulated pattern of miRNA expression (data not shown).
In contrast, Den Hertog et al. (1993) found increased dark respiration of the shoot but observed no change in root respiration in Plantago major when the carbon source was increased by raising the CO2 concentration.
H-HBCD induced Ppara mRNA in mice fed both diets, but we observed no changes in mRNA levels of Lxr (liver X receptor), Rxr (retinoid X receptor), Srebp1 (sterol regulatory element binding transcription factor 1), or Fasn (fatty acid synthase) in either group.
In conclusion, we detected relatively high (micromolar) concentrations of 23 plasma flavanone and phenolic metabolites 5 h after orange juice intake but observed no acute changes in simultaneously assessed cardiovascular risk biomarkers, relative to a sugar and vitamin C matched control.
For example, Piffanelli and colleagues screened 20 Oswrky insertion mutants but observed no phenotypic change for host and non-host pathogen interaction (Berri et al. 2009).
To examine whether this pattern reflected processes of maturation, we asked a panel to re-evaluate several moral dilemmas after an eight-year interval but observed no overall change (Study 2).
