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Of these scaffolds 52 only could be assigned a position but not orientated, either because they carried a single uSNP or because they contained multiple tightly linked SNPs whose map order was questionable due to insufficient radiation-induced breakpoints.
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PCRs with one primer binding in the vector backbone and the other one in the CaMV 35S promoter or smGFP4 coding sequence in our psmGFP4 clearly show that the multiple cloning site is not orientated like in pUC118 and pUC18 but like in pUC119 and pUC19 (data not shown).
Furthermore, sexual activity not orientated toward this good including same-sex activity, masturbation, contracepted sex, sex without marital commitment (even within legal marriage)—is valueless; it does not instantiate any basic good.
SNPs are anchored to genomic scaffolds, which are not orientated or ordered with respect to a genetic map.
The interviewer could request 'priority mode' at any time or it was selected automatically if an individual was not orientated to time or place.
If the specimen was not orientated by the surgeon at the time of operation, a single, non-specified margin measurement was provided.
No superhyperfine splitting is observed in the resulting spectrum which suggests that the fifth ligand does not use nitrogen or the ligand is not orientated directly along the z-axis.
In two of the cases further injections were placed anteriorly, by eye with the needle hand-held, when the manipulator was not orientated optimally to place these injections (two in S2 and three in S4).
"This clearly shows that the beetles do not orientate to a single bright 'lodestar,' but rather to the band of light that represents the Milky Way," the researchers concluded.
Interestingly, when cells are grown in vitro, they do not orientate themselves parallel to the long axis of the tension, but away from it [ 6].
The proportion of activated mosquitoes retrieved from the volunteers' odour-baited trap was 50, 53 and 47% for the treatments BB, BW and AW respectively, indicating that mosquitoes did not orientate preferentially towards human odours relative to outdoor air (figure 2B).
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