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Tamoxifen increased pHi in ER-Ras cells and with NT shRNA but not in vector control cells.
IPA enhances tamoxifen sensitivity in BT474- EBP1-overexpressing cells, but not in vector controls cells (Supplementary Figure 1).
(H ) Treatment with EIPA induces cell death only in sRasV12 expressing cells (2.3× increase over control), but not in vector (0.73×) or control (0.95×) cells.
These results show that Pyk2 promoted an invasive phenotype in PLC cells but not in vector control and PRNK transfectants where the signalling of Pyk2 is being disrupted.
PYK2 was co-immunoprecipitated with exogenous myc tag, which is clearly seen in SOCS-box mutant-transfected A549 cells but not in vector control cells.
PML protein also colocalised with TRF2 and the components of the Mre11/Rad50/Nbs1 (MRN) homologous recombination complex in both HeLa and HT1080 hTR cells, but not in vector controls (Supplementary Figure S2).
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We observed reduced cell viability in GFP vector + APP-sw-nucleofected cells (drop by 36%) but not in GFP vector − or GFP vector + APP-wt-nucleofected cells.
Nonetheless, cell viability dropped significantly in GFP vector + APP-sw-nucleofected cells but not in GFP vector- or GFP vector + APP-wt-nucleofected cells.
The expression of A β 1-16 peptide (any form: A β 1-40, A β 1-42, And A β 1-43) was detected in APP-sw-nucleofected but not in GFP vector- or GFP vector + APP-wt-nucleofected cell cultures).
Luminescence intensity was significantly decreased for the vectors carrying the 3′ UTR and the deletion at position 3353 3359, but not in the vector with the deletion at position 3803 3809, indicating that the region between positions 3803 3809 contains the miR-489 target site.
Both reporters were distinctly upregulated by tomm-40 RNAi), tomm-40 RNAiempty vector" emv(RNAi) controls (Figure 4but
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